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Guangzhou Dongsheng Biotech Co., Ltd
Professional Supplier of PCR & NGS Reagents
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Professional T4 DNA Polymerase #K011

Guangzhou Dongsheng Biotech Co., Ltd
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Professional T4 DNA Polymerase #K011

Brand Name : GDSBio

Model Number : K011

Certification : /

Place of Origin : China

MOQ : 100U

Price : inquiry

Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability : 100 Bag/Bags Per Day

Delivery Time : 8 work days

Packaging Details : small package or bulk distribute or OEM

Cat. No. : K011-A

Specification : 100U

Appearance : colourless

Application : DNA end repair

Classification : General Reagents

Grade : molecular biology

Logo Printing : With Logo Printing

Transport Package : Packing

Production Capacity : 100 Bag/Bags Per Day

Storage Conditions : Store at -20°C

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Cat. No./Spec.: K011-A/100U; K011-B/500U; K011-C/2000U; K011-D/5000U

Concentration: 5U/µL

Product Description

In the presence of templates and primers, T4 DNA Polymerase catalyzes DNA synthesis in the 5 ́→3 ́ direction. This enzyme also has the activity of 3 ́→5 ́ exonuclease which is stronger than E. coli DNA polymerase I. Unlike DNA polymerase I, T4 DNA Polymerase does not have 5 ́→3 ́ exonuclease activity.

Components

Component K011-A (100U) K011-B (500U) K011-C (2,000U) K011-D (5,000U)
T4 DNA Polymerase (5U/µL) 20 µL 100 µL 400 µL 1 mL
10X Blue Buffer 100 µL 250 µL 500 µL 1.25 mL

Storage Condition

All reagents should be stored at -20°C. The product is valid for 12 months.

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.

Scope of Application

1. Removal of 3’ overhangs to form blunt ends;

  • Fill-in of 5’ overhangs to form blunt ends;
  • Labeling DNA probe synthesis through displacement reaction;
  • Synthesis of the second strand during site-directed mutagenesis;
  • PCR product cloning without relying on ligation reactions.

Product Tags:

K011 T4 DNA Polymerase

      

T4 DNA Polymerase

      
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