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Brand Name : GDSBio
Model Number : K010
Certification : ISO9001, ISO13485
Place of Origin : China
MOQ : 100U
Price : USD 7.1-17/100U
Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability : 10000 Box/Boxes per Day
Delivery Time : 8 work days
Packaging Details : small package or bulk distribute or OEM
Cat. No. : K010
Specification : K010-A/100U; K010-B/200U; K010-C/1,000U
Application : ngs library preparation
Stock : yes
Appearance : clear liquid
Logo Printing : With Logo Printing
Transport Package : Packing
Shelf Life : 24 months
Storage Conditions : Store unopened at –15°C to –25°C
Cat. No./Spec.: K010-A/100U; K010-B/200U; K010-C/1,000U
Concentration: 5U/µL
Product Description
Klenow Fragment (3´→ 5´ exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has mutations which abolish the 3´→ 5´ exonuclease activity.
Components
| Component | K010-A (100U) | K010-B (200U) | K010-C (1,000U) |
| Klenow Fragment(3´→5´ exo-)(5U/µL) | 20 µL | 40 µL | 200 µL |
| 10X Klenow Buffer | 1.25 mL | 1.25 mL | 1.25 mL |
Storage Condition & Shelf Life
All reagents should be stored at -20°C. The product is valid for 24 months.
Source
Recombinant E. coli strain.
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
Scope of Application
1. Generates probes using random primers
2. Random priming labeling
3. dA-tailing at 3' end
4. Second strand cDNA synthesis
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Klenow Fragment 3-5 exo- #K010 Images |