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Guangzhou Dongsheng Biotech Co., Ltd
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Klenow Fragment 3-5 exo- #K010

Guangzhou Dongsheng Biotech Co., Ltd
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Klenow Fragment 3-5 exo- #K010

Brand Name : GDSBio

Model Number : K010

Certification : ISO9001, ISO13485

Place of Origin : China

MOQ : 100U

Price : USD 7.1-17/100U

Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability : 10000 Box/Boxes per Day

Delivery Time : 8 work days

Packaging Details : small package or bulk distribute or OEM

Cat. No. : K010

Specification : K010-A/100U; K010-B/200U; K010-C/1,000U

Application : ngs library preparation

Stock : yes

Appearance : clear liquid

Logo Printing : With Logo Printing

Transport Package : Packing

Shelf Life : 24 months

Storage Conditions : Store unopened at –15°C to –25°C

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Cat. No./Spec.: K010-A/100U; K010-B/200U; K010-C/1,000U

Concentration: 5U/µL

Product Description

Klenow Fragment (3´→ 5´ exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has mutations which abolish the 3´→ 5´ exonuclease activity.

Components

Component K010-A (100U) K010-B (200U) K010-C (1,000U)
Klenow Fragment(3´→5´ exo-)(5U/µL) 20 µL 40 µL 200 µL
10X Klenow Buffer 1.25 mL 1.25 mL 1.25 mL

Storage Condition & Shelf Life

All reagents should be stored at -20°C. The product is valid for 24 months.

Source

Recombinant E. coli strain.

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.

Scope of Application

1. Generates probes using random primers

2. Random priming labeling

3. dA-tailing at 3' end

4. Second strand cDNA synthesis


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