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Fast T4 DNA Ligase (Fast) #K012

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Guangzhou Dongsheng Biotech Co., Ltd

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Fast T4 DNA Ligase (Fast) #K012

Name: Fast T4 DNA Ligase Fast K012
Brand: GDSBio
Price: 1 USD

Brand Name : GDSBio

Model Number : K012

Certification : /

Place of Origin : China

MOQ : 1000U

Price : inquiry

Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability : 100 Bag/Bags Per Day

Delivery Time : 8 work days

Packaging Details : small package or bulk distribute or OEM

Cat. No. : K012

Specification : 1000U

Appearance : colourless

Application : DNA ligation

Classification : General Reagents

Grade : molecular biology

Logo Printing : With Logo Printing

Transport Package : Packing

Production Capacity : 100 Bag/Bags Per Day

Storage Conditions : Store at -20°C

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Cat. No./Spec.: K012/1000 U

Concentration: 5 U/µL

Note: 1 U=1 Weiss unit

Product Description

T4 DNA Ligase (Fast) is produced by E. coli carrying T4 bacteriophage gene 30. This enzyme catalyzes the formation of phosphodiester bonds between the 5'-phosphate group and the 3'-hydroxyl group of double-helix DNA or RNA. This enzyme can repair single-strand breaks in double-stranded DNA, RNA, or DNA/RNA complexes, and can connect DNA fragments with sticky or blunt ends, but it is inactive for single-stranded nucleic acids. It is mainly used for cloning of restriction enzyme digestion products, gene site-directed mutagenesis, PCR product cloning, linear DNA self-looping, and repair of double-stranded DNA breaks. T4 DNA Ligase (Fast) requires ATP as a cofactor and completes the sticky end ligation reaction at room temperature in only 10 minutes.

Components

Component	K012 (1,000 U)
T4 DNA Ligase (Fast) (5 U/µL)	200 µL
10X T4 DNA Ligase Buffer	1 mL
50% PEG	1 mL

Storage Condition

Store at -20°C.

Unit Definition

At 37°C, 1 Weiss unit of enzyme catalyzes the conversion of 1 nmol of [³²PPi] into an activated carbon adsorption state within 20 minutes. One Weiss unit is equivalent to approximately 200 cohesive end ligation units (CEU), which is equivalent to the number of HindIII digested DNA fragments that can be ligated within 30 minutes at 16°C. λ DNA fragments.

Product Tags:

T4 DNA Ligase fast

Fast T4 DNA Ligase

Fast T4 DNA Ligase (Fast) #K012 Images

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