General RNA Extraction Kit
Catalog No.: R1051 (50 preps)
Kit Contents
| Component | R1051 |
| 50 preps |
| Solution RL | 60 ml |
| Wash Buffer RPI | 18 ml |
| Wash Buffer RW | 12 ml |
| DEPC-treated water | 10 ml |
| RNase-free Spin Column | 50 each |
| RNase-free Microcentrifuge Tube | 50 each |
Materials supplied by the users:
• Chloroform
• Ethanol (96–100%)
Description
General RNA Extraction Kit provides a simple method of isolating total RNA from a wide range of sample types and amounts. In general, samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt which is capable of protecting the RNA from endogenous RNases. After homogenization, ethanol is added to the sample. The sample is then processed through a spin column containing a clear silica-based membrane where the RNA binds. Any impurities are effectively removed by subsequent washing. The purified total RNA is then eluted in RNase-Free Water and is suitable for use in a variety of downstream applications.
Downstream Applications
Purified RNA is free of impurities and enzyme inhibitors, and have an A260/280=1.8-2.0, is suitable for applications such as:
• Reverse transcription PCR (RT-PCR)
• Real-time quantitative PCR (qPCR)
• Northern blotting
• Nuclease protection assays
• RNA amplification for microarray analysis
• cDNA library preparation after poly(A)+ selection
Features
• Stable yield
• Reliable performance of high-quality purified total RNA in downstream applications
Storage
DEPC-treated water store at -20 ̊C, other reagents store at 2-8 ̊C, protect from light. Kit contents are stable for up to 12 months when stored properly.
