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Fast DNA Library Prep Kit For High-throughput Sequencing Platform

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Guangzhou Dongsheng Biotech Co., Ltd

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Fast DNA Library Prep Kit For High-throughput Sequencing Platform

Name: Fast DNA Library Prep Kit For Highthroughput Sequencing Platform
Brand: GDSBio
Price: 1 USD

Brand Name : GDSBio

Model Number : K001-A, K001-B

Certification : ISO9001, ISO13485

Place of Origin : China

MOQ : 6 rxns

Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability : 500000Kits/Week

Delivery Time : 8work days

Packaging Details : Carton Packaging

Package Size : K001-A/24 rxns; K001-B/96 rxns; sample sack/ 6 rxns

Storage : Stored at 5 ~ 25°C, with a validity period of 12 months

Adapters : Standard, Customized

Delivery Format : Plates, Tubes

End Repair : Yes

Fragmentation Method : Ultrasonic, Enzymatic

Input Amount : 10ng-1ug

Input Type : Plasmid, PCR Product, Genomic DNA, Total RNA

Library Normalization : Yes

Library Quality Control : Qubit, Bioanalyzer, qPCR

Library Quantification : Qubit, qPCR

Library Type : DNA/RNA

Product Name : NGS Library Construction

Service Support : Yes

Size Selection : Yes

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Fast DNA Library Prep Kit

Instruction for Use

【Product Name】

Fast DNA Library Prep Kit

【Cat. No./Spec.】

K001-A/24 rxns; K001-B/96 rxns; sample sack/ 6 rxns

【Product Description】

Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, and reagents are highly premixed, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

【Sample Type】

Application	Sample Type	Recommended Amount
Whole genome sequencing	High quality complex genomes	50ng-1μg
Target capture sequencing of whole exome	High quality complex genomes	10ng-1μg
Target capture sequencing of whole genome	FFPE DNA	≥50ng
Target capture sequencing of whole genome	cfDNA/ctDNA	≥100pg
Whole genome sequencing	Microbial genome	1ng-1μg
Whole genome sequencing(PCR-free)	High quality DNA	≥50ng (no size selection) ≥200ng (size selection)
ChIP-Seq	ChIP DNA	≥100pg
Targeted sequencing	Amplicon	≥100pg

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.

【Components】

Component	24 rxns	96 rxns
End Repair & A-Tailing Mix	480 μl	4×480 μl
Fast DNA Ligase	120 μl	2×240 μl
Fast Ligation Buffer	600 μl	4×600 μl
2× HIFI Library PCR Master Mix	600 μl	4×600 μl
Primer Mix*	120 μl	480 μl

* If there are more than one sample, #K002 and #K003 adapter primer mix is recommended. This kit provides a set of primers with index.

Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.

Patented formula, contains highly efficient lysate for rapid lysis and inactivation of virus, and eliminate biosafety hazards
Fast DNA Library Prep Kit For High-throughput Sequencing Platform

Fast DNA Library Prep Kit For High-throughput Sequencing Platform

Environmentally friendly and strong carton packing

Fast DNA Library Prep Kit For High-throughput Sequencing Platform

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